Strategies for Establishing and Utilizing a Secure Cell Line Expressing Each Gaussia Luciferase and Firefly Luciferase to Display screen for Endoplasmic Reticulum Stress.
- Coiled-coil area containing 67 (CCDC67) gene is a tumor suppressor gene that displays a major inhibitory impact on quite a lot of tumors. Our earlier research demonstrated that the upregulation of CCDC67 gene in TPC-1 cells inhibited cell proliferation, migration and invasion, and promoted apoptosis in vitro.
- Nevertheless, because of the lack of a appropriate cell software, these outcomes weren’t validated in vivo. Within the current research, a thyroid most cancers cell line with steady expression of CCDC67 and luciferase reporter genes was generated and recognized. Firstly, cDNA clones of the CCDC67 gene had been obtained by reverse transcription utilizing a custom-designed primer.
- The outcomes of subsequent electrophoresis evaluation and sequencing revealed that the cDNA clones of CCDC67 gene had been obtained efficiently, with a size of 1,862 bp. The lentiviral vectors, containing the CCDC67, luciferase reporter and puromycin acetyltransferase genes, had been co-transfected with two plasmids that encode lentiviral structural proteins and envelope proteins into 293T cells.
AFAP1L2 3’UTR Luciferase Secure Cell Line
- Following ultracentrifugation, the titer of lentivirus was decided by ELISA to be 5.0×108 TU/ml. The constructed lentiviral vector was used to transfect TPC-1 thyroid most cancers cells, and stabilization was achieved by puromycin screening. The expression of CCDC67 gene, luciferase exercise and tumorigenic means of the generated cell line had been detected.
- Reverse transcription-qPCR outcomes demonstrated that the expression ranges of CCDC67 gene in TPC-1 cells following transfection had been elevated 194,46.782-fold in contrast with these within the unfavourable management group (P<0.01).
- A better fluorescence depth was detected within the generated cell line, whereas no detectable fluorescence was noticed in untransfected TPC-1 cells. The tumorigenic means of TPC-1-Luc-Puromycin-CCDC67 cells was verified by bioluminescence imaging and histopathological evaluation utilizing a pulmonary metastasis mannequin.
Human Angiotensinogen (AGT) ELISA Equipment | Mouse Angiotensinogen (AGT) ELISA Equipment
- These outcomes demonstrated {that a} thyroid most cancers cell line with steady expression of CCDC67 and luciferase reporter genes was generated efficiently. The TPC-1-Luc-Puromycin-CCDC67 cell line could also be a useful software for additional analysis on CCDC67 in vivo.
SERPINE2 3'UTR Luciferase Stable Cell Line |
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TU022972 | ABM | 1.0 ml | EUR 1672.8 |
Serpine2 3'UTR GFP Stable Cell Line |
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TU270187 | ABM | 1.0 ml | Ask for price |
SERPINE2 3'UTR GFP Stable Cell Line |
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TU072972 | ABM | 1.0 ml | EUR 1672.8 |
Serpine2 3'UTR GFP Stable Cell Line |
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TU168642 | ABM | 1.0 ml | Ask for price |
A1CF 3'UTR Luciferase Stable Cell Line |
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TU000003 | ABM | 1.0 ml | EUR 1672.8 |
A2LD1 3'UTR Luciferase Stable Cell Line |
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TU000004 | ABM | 1.0 ml | EUR 1672.8 |
A2M 3'UTR Luciferase Stable Cell Line |
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TU000005 | ABM | 1.0 ml | EUR 1672.8 |
A2ML1 3'UTR Luciferase Stable Cell Line |
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TU000006 | ABM | 1.0 ml | EUR 1672.8 |
A2MP1 3'UTR Luciferase Stable Cell Line |
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TU000007 | ABM | 1.0 ml | EUR 2799.6 |
A3GALT2 3'UTR Luciferase Stable Cell Line |
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TU000008 | ABM | 1.0 ml | EUR 2799.6 |
A4GALT 3'UTR Luciferase Stable Cell Line |
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TU000009 | ABM | 1.0 ml | EUR 1672.8 |
A4GNT 3'UTR Luciferase Stable Cell Line |
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TU000010 | ABM | 1.0 ml | EUR 1672.8 |
AAAS 3'UTR Luciferase Stable Cell Line |
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TU000011 | ABM | 1.0 ml | EUR 1672.8 |
AACP 3'UTR Luciferase Stable Cell Line |
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TU000012 | ABM | 1.0 ml | EUR 2799.6 |
AACS 3'UTR Luciferase Stable Cell Line |
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TU000013 | ABM | 1.0 ml | EUR 1672.8 |
AACSL 3'UTR Luciferase Stable Cell Line |
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TU000014 | ABM | 1.0 ml | EUR 2799.6 |
AADAC 3'UTR Luciferase Stable Cell Line |
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TU000015 | ABM | 1.0 ml | EUR 1672.8 |
AADACL2 3'UTR Luciferase Stable Cell Line |
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TU000016 | ABM | 1.0 ml | EUR 2799.6 |
AADACL3 3'UTR Luciferase Stable Cell Line |
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TU000017 | ABM | 1.0 ml | EUR 1825.2 |
AADACL4 3'UTR Luciferase Stable Cell Line |
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TU000018 | ABM | 1.0 ml | EUR 1672.8 |
AADAT 3'UTR Luciferase Stable Cell Line |
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TU000019 | ABM | 1.0 ml | EUR 1672.8 |
AAGAB 3'UTR Luciferase Stable Cell Line |
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TU000020 | ABM | 1.0 ml | EUR 2799.6 |
AAK1 3'UTR Luciferase Stable Cell Line |
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TU000021 | ABM | 1.0 ml | EUR 1825.2 |
AAMP 3'UTR Luciferase Stable Cell Line |
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TU000022 | ABM | 1.0 ml | EUR 1672.8 |
AANAT 3'UTR Luciferase Stable Cell Line |
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TU000023 | ABM | 1.0 ml | EUR 1672.8 |
AARS 3'UTR Luciferase Stable Cell Line |
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TU000024 | ABM | 1.0 ml | EUR 1672.8 |
AARS2 3'UTR Luciferase Stable Cell Line |
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TU000025 | ABM | 1.0 ml | EUR 1672.8 |
AARSD1 3'UTR Luciferase Stable Cell Line |
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TU000026 | ABM | 1.0 ml | EUR 1672.8 |
AASDH 3'UTR Luciferase Stable Cell Line |
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TU000027 | ABM | 1.0 ml | EUR 1825.2 |
AASDHPPT 3'UTR Luciferase Stable Cell Line |
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TU000028 | ABM | 1.0 ml | EUR 1825.2 |
AASS 3'UTR Luciferase Stable Cell Line |
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TU000029 | ABM | 1.0 ml | EUR 2799.6 |
AATF 3'UTR Luciferase Stable Cell Line |
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TU000030 | ABM | 1.0 ml | EUR 1672.8 |
AATK 3'UTR Luciferase Stable Cell Line |
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TU000031 | ABM | 1.0 ml | EUR 1825.2 |
ABAT 3'UTR Luciferase Stable Cell Line |
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TU000033 | ABM | 1.0 ml | EUR 2799.6 |
ABCA1 3'UTR Luciferase Stable Cell Line |
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TU000034 | ABM | 1.0 ml | EUR 2799.6 |
ABCA2 3'UTR Luciferase Stable Cell Line |
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TU000035 | ABM | 1.0 ml | EUR 5540.4 |
ABCA3 3'UTR Luciferase Stable Cell Line |
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TU000036 | ABM | 1.0 ml | EUR 5540.4 |
ABCA4 3'UTR Luciferase Stable Cell Line |
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TU000037 | ABM | 1.0 ml | EUR 1672.8 |
ABCA5 3'UTR Luciferase Stable Cell Line |
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TU000038 | ABM | 1.0 ml | EUR 2799.6 |
ABCA6 3'UTR Luciferase Stable Cell Line |
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TU000039 | ABM | 1.0 ml | EUR 2799.6 |
ABCA7 3'UTR Luciferase Stable Cell Line |
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TU000040 | ABM | 1.0 ml | EUR 1672.8 |
ABCA8 3'UTR Luciferase Stable Cell Line |
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TU000041 | ABM | 1.0 ml | EUR 2799.6 |
ABCA9 3'UTR Luciferase Stable Cell Line |
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TU000042 | ABM | 1.0 ml | EUR 1825.2 |
ABCA10 3'UTR Luciferase Stable Cell Line |
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TU000043 | ABM | 1.0 ml | EUR 2799.6 |
ABCA12 3'UTR Luciferase Stable Cell Line |
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TU000045 | ABM | 1.0 ml | EUR 1672.8 |
ABCA13 3'UTR Luciferase Stable Cell Line |
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TU000046 | ABM | 1.0 ml | EUR 2799.6 |
ABCB1 3'UTR Luciferase Stable Cell Line |
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TU000048 | ABM | 1.0 ml | EUR 2799.6 |
ABCB4 3'UTR Luciferase Stable Cell Line |
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TU000049 | ABM | 1.0 ml | EUR 1672.8 |
ABCB5 3'UTR Luciferase Stable Cell Line |
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TU000050 | ABM | 1.0 ml | EUR 1672.8 |
ABCB6 3'UTR Luciferase Stable Cell Line |
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TU000051 | ABM | 1.0 ml | EUR 1825.2 |
ABCB7 3'UTR Luciferase Stable Cell Line |
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TU000052 | ABM | 1.0 ml | EUR 1825.2 |
ABCB8 3'UTR Luciferase Stable Cell Line |
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TU000053 | ABM | 1.0 ml | EUR 2799.6 |
ABCB9 3'UTR Luciferase Stable Cell Line |
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TU000054 | ABM | 1.0 ml | EUR 1672.8 |
ABCB10 3'UTR Luciferase Stable Cell Line |
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TU000055 | ABM | 1.0 ml | EUR 1825.2 |
ABCB10P1 3'UTR Luciferase Stable Cell Line |
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TU000056 | ABM | 1.0 ml | EUR 2799.6 |
ABCB10P2 3'UTR Luciferase Stable Cell Line |
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TU000057 | ABM | 1.0 ml | EUR 2799.6 |
ABCB10P3 3'UTR Luciferase Stable Cell Line |
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TU000058 | ABM | 1.0 ml | EUR 2799.6 |
ABCB10P4 3'UTR Luciferase Stable Cell Line |
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TU000059 | ABM | 1.0 ml | EUR 2799.6 |
ABCB11 3'UTR Luciferase Stable Cell Line |
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TU000060 | ABM | 1.0 ml | EUR 1825.2 |
ABCC1 3'UTR Luciferase Stable Cell Line |
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TU000061 | ABM | 1.0 ml | EUR 1825.2 |
Hepa1-6-FLuc cell line with the steady expression of firefly luciferase retains its main properties with promising bioluminescence imaging means.
- Dependable animal fashions are required for the in vivo research of the molecular mechanisms and results of chemotherapeutic medicine in hepatocarcinoma.
- In vivo tracing methods based mostly on firefly luciferase (FLuc) could optimize the non-invasive monitoring of experimental animals. The current research established a murine Hepa1-6-FLuc cell line that stably expressed a retrovirus-delivered FLuc protein gene.
Rat Angiotensinogen (AGT) ELISA Equipment | Mouse Angiotensinogen (AGT) ELISA Equipment
- The cell morphology, proliferation, migration and invasion means of Hepa1-6-FLuc cells had been the identical as that of the Hepa1-6 cells, and thus is appropriate to exchange Hepa1-6 cells within the building of hepatocarcinoma animal fashions. No variations in subcutaneous tumor mass and its pathomorphology from implanted Hepa1-6-FLuc cells had been noticed in contrast with Hepa1-6 management tumors.
Human Angiotensinogen (AGT) ELISA Kit |
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RD-AGT-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 501.6 |
Human Angiotensinogen (AGT) ELISA Kit |
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RD-AGT-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 690 |
Mouse Angiotensinogen (AGT) ELISA Kit |
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RD-AGT-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 514.8 |
Mouse Angiotensinogen (AGT) ELISA Kit |
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RD-AGT-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 709.2 |
Rat Angiotensinogen (AGT) ELISA Kit |
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RD-AGT-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 540 |
Rat Angiotensinogen (AGT) ELISA Kit |
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RD-AGT-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 746.4 |
Human Angiotensinogen (AGT) ELISA Kit |
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RDR-AGT-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 523.2 |
Human Angiotensinogen (AGT) ELISA Kit |
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RDR-AGT-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 721.2 |
Mouse Angiotensinogen (AGT) ELISA Kit |
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RDR-AGT-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 536.4 |
Mouse Angiotensinogen (AGT) ELISA Kit |
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RDR-AGT-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 741.6 |
Rat Angiotensinogen (AGT) ELISA Kit |
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RDR-AGT-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 564 |
Rat Angiotensinogen (AGT) ELISA Kit |
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RDR-AGT-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 781.2 |
AGT antibody |
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70R-14180 | Fitzgerald | 100 ug | EUR 386.4 |
Description: Affinity purified Rabbit polyclonal AGT antibody |
AGT antibody |
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70R-15631 | Fitzgerald | 50 ul | EUR 522 |
Description: Rabbit polyclonal AGT antibody |
AGT antibody |
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38789-100ul | SAB | 100ul | EUR 302.4 |
AGT Antibody |
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36739-100ul | SAB | 100ul | EUR 302.4 |
AGT antibody |
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10R-1811 | Fitzgerald | 100 ul | EUR 483.6 |
Description: Mouse monoclonal AGT antibody |
AGT Antibody |
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ABD7976 | Lifescience Market | 100 ug | EUR 525.6 |
AGT Antibody |
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ABD8022 | Lifescience Market | 100 ug | EUR 525.6 |
AGT Antibody |
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ABD8094 | Lifescience Market | 100 ug | EUR 525.6 |
AGT antibody |
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70R-6214 | Fitzgerald | 50 ug | EUR 560.4 |
Description: Rabbit polyclonal AGT antibody |
AGT Antibody |
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BF0069 | Affbiotech | 200ul | EUR 540 |
AGT Antibody |
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1-CSB-PA001463EA01HU | Cusabio |
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Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000 |
AGT Antibody |
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1-CSB-PA001463ESR1HU | Cusabio |
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Description: A polyclonal antibody against AGT. Recognizes AGT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200 |
AGT Antibody |
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1-CSB-PA001463ESR2HU | Cusabio |
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Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
AGT Antibody |
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1-CSB-PA001463GA01HU | Cusabio |
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Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC |
AGT Antibody |
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1-CSB-PA446811 | Cusabio |
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Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100 |
AGT Antibody |
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1-CSB-PA090852 | Cusabio |
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Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:5000, WB:1:500-1:2000 |
AGT Antibody |
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1-CSB-PA693559 | Cusabio |
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Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:1000-1:5000, IHC:1:25-1:100 |
AGT Antibody |
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1-CSB-PA959270 | Cusabio |
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Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100 |
AGT Antibody |
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1-CSB-PA982509 | Cusabio |
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Description: A polyclonal antibody against AGT. Recognizes AGT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:1000-1:2000, WB:1:200-1:1000 |
AGT Antibody |
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1-CSB-PA173097 | Cusabio |
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Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA;ELISA:1:1000-1:2000 |
AGT Antibody |
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1-CSB-PA10079A0Rb | Cusabio |
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Description: A polyclonal antibody against AGT. Recognizes AGT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:400, IF:1:50-1:200, IP:1:200-1:2000 |
AGT Antibody |
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1-CSB-PA281661 | Cusabio |
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Description: A polyclonal antibody against AGT. Recognizes AGT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:5000, WB:1:500-1:2000 |
AGT Antibody |
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DF7976 | Affbiotech | 200ul | EUR 420 |
Angiotensinogen (AGT) Antibody |
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20-abx171240 | Abbexa |
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Angiotensinogen (AGT) Antibody |
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20-abx101137 | Abbexa |
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Angiotensinogen (AGT) Antibody |
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20-abx101138 | Abbexa |
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Angiotensinogen (AGT) Antibody |
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20-abx101139 | Abbexa |
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Angiotensinogen (AGT) Antibody |
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20-abx101140 | Abbexa |
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Angiotensinogen (AGT) Antibody |
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20-abx004798 | Abbexa |
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Angiotensinogen (AGT) Antibody |
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20-abx128540 | Abbexa |
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Angiotensinogen (AGT) Antibody |
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20-abx110982 | Abbexa |
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Angiotensinogen (AGT) Antibody |
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abx010360-100ul | Abbexa | 100 ul | EUR 493.2 |
Angiotensinogen (AGT) Antibody |
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abx033720-400ul | Abbexa | 400 ul | EUR 627.6 |
Angiotensinogen (AGT) Antibody |
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abx033720-80l | Abbexa | 80 µl | EUR 343.2 |
Angiotensinogen (AGT) Antibody |
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abx033721-400ul | Abbexa | 400 ul | EUR 627.6 |
Angiotensinogen (AGT) Antibody |
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abx033721-80l | Abbexa | 80 µl | EUR 343.2 |
Angiotensinogen (AGT) Antibody |
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20-abx320213 | Abbexa |
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Angiotensinogen (AGT) Antibody |
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20-abx322250 | Abbexa |
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Angiotensinogen (AGT) Antibody |
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abx230393-100ug | Abbexa | 100 ug | EUR 610.8 |
Angiotensinogen (AGT) Antibody |
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abx230394-100ug | Abbexa | 100 ug | EUR 577.2 |
Angiotensinogen (AGT) Antibody |
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abx230395-100ug | Abbexa | 100 ug | EUR 577.2 |
Angiotensinogen (AGT) Antibody |
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20-abx241726 | Abbexa |
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Angiotensinogen (AGT) Antibody |
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20-abx241946 | Abbexa |
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Angiotensinogen (AGT) Antibody |
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20-abx241947 | Abbexa |
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Angiotensinogen (AGT) Antibody |
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20-abx242324 | Abbexa |
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Angiotensinogen (AGT) Antibody |
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20-abx301357 | Abbexa |
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Angiotensinogen (AGT) Antibody |
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20-abx339646 | Abbexa |
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Angiotensinogen (AGT) Antibody |
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20-abx211344 | Abbexa |
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Angiotensinogen (AGT) Antibody |
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20-abx214416 | Abbexa |
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Angiotensinogen (AGT) Antibody |
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20-abx175400 | Abbexa |
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AGT Conjugated Antibody |
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C36739 | SAB | 100ul | EUR 476.4 |
AGT Conjugated Antibody |
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C38789 | SAB | 100ul | EUR 476.4 |
Anti-AGT antibody |
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STJ118098 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-AGT antibody |
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STJ97818 | St John's Laboratory | 100 µl | EUR 280.8 |
Description: Mouse monoclonal to AGT. |
Anti-AGT antibody |
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STJ28201 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. |
AGT Polyclonal Antibody |
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A50656-020 | EpiGentek | 20 ul | EUR 117.7 |
AGT Polyclonal Antibody |
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A50656-050 | EpiGentek | 50 ul | EUR 302.5 |
AGT Polyclonal Antibody |
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A50656-100 | EpiGentek | 100 ul | EUR 423.5 |
AGT Polyclonal Antibody |
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A70590-050 | EpiGentek | 50 ul | EUR 302.5 |
AGT Polyclonal Antibody |
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A70590-100 | EpiGentek | 100 ul | EUR 423.5 |
AGT Polyclonal Antibody |
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A50656 | EpiGentek |
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AGT Polyclonal Antibody |
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A70590 | EpiGentek |
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AGT Antibody (CT) |
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6708-100 | Biovision | each | EUR 379.2 |
AGT Antibody (NT) |
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6709-100 | Biovision | each | EUR 379.2 |
Agt/ Rat Agt ELISA Kit |
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ELI-02702r | Lifescience Market | 96 Tests | EUR 1063.2 |
aGT/ Rat aGT ELISA Kit |
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ELA-E0797r | Lifescience Market | 96 Tests | EUR 1063.2 |
AGT siRNA |
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20-abx907049 | Abbexa |
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AGT siRNA |
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20-abx907050 | Abbexa |
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Anti-Angiotensinogen/AGT Antibody |
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A02103-1 | BosterBio | 100ug/vial | EUR 400.8 |
AGT/Angiotensin-1 Antibody |
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47494-100ul | SAB | 100ul | EUR 302.4 |
Angiotensinogen (AGT) Antibody Pair |
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20-abx370425 | Abbexa |
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Angiotensinogen (AGT) Antibody Pair |
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20-abx370604 | Abbexa |
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Angiotensinogen (AGT) Antibody (FITC) |
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20-abx273972 | Abbexa |
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Angiotensinogen (AGT) Antibody (FITC) |
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20-abx274115 | Abbexa |
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Angiotensinogen (AGT) Antibody (Biotin) |
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20-abx272741 | Abbexa |
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Angiotensinogen (AGT) Antibody (Biotin) |
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20-abx273211 | Abbexa |
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Angiotensinogen (AGT) Antibody (HRP) |
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20-abx306453 | Abbexa |
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Angiotensinogen (AGT) Antibody (FITC) |
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20-abx306454 | Abbexa |
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- Bioluminescence imaging indicated that the Luc sign of the Hepa1-6-FLuc cells was constantly strengthened with will increase in tumor mass; nonetheless, the Luc sign of Hepa1-6-AdFLuc turned weaker and finally disappeared throughout tumor improvement.
- Subsequently, in contrast with the transient expression by adenovirus, steady expression of the FLuc gene in Hepa1-6 cells could higher replicate cell proliferation and survival in vivo, and supply a dependable supply for the institution of hepatocarcinoma fashions.
- Bioluminescent tumor cell strains are experimental instruments of main significance for most cancers investigation, particularly imaging of tumors in xenografted animals. Secure expression of exogenous luciferase in tumor cells mixed to systemic injection of luciferin supplies a wonderful sign/background ratio for exterior optical imaging. Subsequently, there’s a have to rationalize and pace up the manufacturing of luciferase-positive tumor cell strains consultant of a number of tumor phenotypes.