Top Luciferase Stable Cell Line

Top Luciferase Stable Cell Line

Strategies for Establishing and Utilizing a Secure Cell Line Expressing Each Gaussia Luciferase and Firefly Luciferase to Display screen for Endoplasmic Reticulum Stress.

 

  • Coiled-coil area containing 67 (CCDC67) gene is a tumor suppressor gene that displays a major inhibitory impact on quite a lot of tumors. Our earlier research demonstrated that the upregulation of CCDC67 gene in TPC-1 cells inhibited cell proliferation, migration and invasion, and promoted apoptosis in vitro.

 

  • Nevertheless, because of the lack of a appropriate cell software, these outcomes weren’t validated in vivo. Within the current research, a thyroid most cancers cell line with steady expression of CCDC67 and luciferase reporter genes was generated and recognized. Firstly, cDNA clones of the CCDC67 gene had been obtained by reverse transcription utilizing a custom-designed primer.

 

  • The outcomes of subsequent electrophoresis evaluation and sequencing revealed that the cDNA clones of CCDC67 gene had been obtained efficiently, with a size of 1,862 bp. The lentiviral vectors, containing the CCDC67, luciferase reporter and puromycin acetyltransferase genes, had been co-transfected with two plasmids that encode lentiviral structural proteins and envelope proteins into 293T cells.

        AFAP1L2 3’UTR Luciferase Secure Cell Line

  • Following ultracentrifugation, the titer of lentivirus was decided by ELISA to be 5.0×108 TU/ml. The constructed lentiviral vector was used to transfect TPC-1 thyroid most cancers cells, and stabilization was achieved by puromycin screening. The expression of CCDC67 gene, luciferase exercise and tumorigenic means of the generated cell line had been detected.

 

  • Reverse transcription-qPCR outcomes demonstrated that the expression ranges of CCDC67 gene in TPC-1 cells following transfection had been elevated 194,46.782-fold in contrast with these within the unfavourable management group (P<0.01).

 

  • A better fluorescence depth was detected within the generated cell line, whereas no detectable fluorescence was noticed in untransfected TPC-1 cells. The tumorigenic means of TPC-1-Luc-Puromycin-CCDC67 cells was verified by bioluminescence imaging and histopathological evaluation utilizing a pulmonary metastasis mannequin.

           Human Angiotensinogen (AGT) ELISA Equipment | Mouse Angiotensinogen (AGT) ELISA Equipment

  • These outcomes demonstrated {that a} thyroid most cancers cell line with steady expression of CCDC67 and luciferase reporter genes was generated efficiently. The TPC-1-Luc-Puromycin-CCDC67 cell line could also be a useful software for additional analysis on CCDC67 in vivo.

Serpine2 3'UTR Luciferase Stable Cell Line

TU220187 1.0 ml Ask for price

SERPINE1 3'UTR Luciferase Stable Cell Line

TU022971 1.0 ml
EUR 1825.2

SERPINE3 3'UTR Luciferase Stable Cell Line

TU022973 1.0 ml
EUR 1672.8

Serpine1 3'UTR Luciferase Stable Cell Line

TU118641 1.0 ml Ask for price

Serpine3 3'UTR Luciferase Stable Cell Line

TU118643 1.0 ml Ask for price

Serpine1 3'UTR Luciferase Stable Cell Line

TU220186 1.0 ml Ask for price

Serpine3 3'UTR Luciferase Stable Cell Line

TU220188 1.0 ml Ask for price

SERPINA1 3'UTR Luciferase Stable Cell Line

TU022943 1.0 ml
EUR 1825.2

SERPINA2 3'UTR Luciferase Stable Cell Line

TU022944 1.0 ml
EUR 2799.6

SERPINA3 3'UTR Luciferase Stable Cell Line

TU022945 1.0 ml
EUR 1825.2

SERPINA4 3'UTR Luciferase Stable Cell Line

TU022946 1.0 ml
EUR 1672.8

SERPINA5 3'UTR Luciferase Stable Cell Line

TU022947 1.0 ml
EUR 1672.8

SERPINA6 3'UTR Luciferase Stable Cell Line

TU022948 1.0 ml
EUR 1672.8

SERPINA7 3'UTR Luciferase Stable Cell Line

TU022949 1.0 ml
EUR 1672.8

SERPINA9 3'UTR Luciferase Stable Cell Line

TU022950 1.0 ml
EUR 1672.8

SERPINB1 3'UTR Luciferase Stable Cell Line

TU022955 1.0 ml
EUR 1672.8

SERPINB2 3'UTR Luciferase Stable Cell Line

TU022956 1.0 ml
EUR 1672.8

SERPINB3 3'UTR Luciferase Stable Cell Line

TU022957 1.0 ml
EUR 1672.8

SERPINB4 3'UTR Luciferase Stable Cell Line

TU022958 1.0 ml
EUR 1672.8

SERPINB5 3'UTR Luciferase Stable Cell Line

TU022959 1.0 ml
EUR 1672.8

SERPINB6 3'UTR Luciferase Stable Cell Line

TU022960 1.0 ml
EUR 2799.6

SERPINB7 3'UTR Luciferase Stable Cell Line

TU022961 1.0 ml
EUR 1672.8

SERPINB8 3'UTR Luciferase Stable Cell Line

TU022962 1.0 ml
EUR 1825.2

SERPINB9 3'UTR Luciferase Stable Cell Line

TU022964 1.0 ml
EUR 1825.2

SERPINC1 3'UTR Luciferase Stable Cell Line

TU022969 1.0 ml
EUR 1672.8

SERPIND1 3'UTR Luciferase Stable Cell Line

TU022970 1.0 ml
EUR 1672.8

SERPINF1 3'UTR Luciferase Stable Cell Line

TU022974 1.0 ml
EUR 1672.8

SERPINF2 3'UTR Luciferase Stable Cell Line

TU022975 1.0 ml
EUR 1672.8

SERPING1 3'UTR Luciferase Stable Cell Line

TU022976 1.0 ml
EUR 2799.6

SERPINH1 3'UTR Luciferase Stable Cell Line

TU022977 1.0 ml
EUR 1672.8

SERPINI1 3'UTR Luciferase Stable Cell Line

TU022979 1.0 ml
EUR 1672.8

SERPINI2 3'UTR Luciferase Stable Cell Line

TU022980 1.0 ml
EUR 1672.8

Serpina5 3'UTR Luciferase Stable Cell Line

TU118608 1.0 ml Ask for price

Serpina6 3'UTR Luciferase Stable Cell Line

TU118609 1.0 ml Ask for price

Serpina7 3'UTR Luciferase Stable Cell Line

TU118610 1.0 ml Ask for price

Serpina9 3'UTR Luciferase Stable Cell Line

TU118611 1.0 ml Ask for price

Serpinb2 3'UTR Luciferase Stable Cell Line

TU118619 1.0 ml Ask for price

Serpinb5 3'UTR Luciferase Stable Cell Line

TU118624 1.0 ml Ask for price

Serpinb7 3'UTR Luciferase Stable Cell Line

TU118630 1.0 ml Ask for price

Serpinb8 3'UTR Luciferase Stable Cell Line

TU118631 1.0 ml Ask for price

Serpinb9 3'UTR Luciferase Stable Cell Line

TU118632 1.0 ml Ask for price

Serpinc1 3'UTR Luciferase Stable Cell Line

TU118639 1.0 ml Ask for price

Serpind1 3'UTR Luciferase Stable Cell Line

TU118640 1.0 ml Ask for price

Serpinf1 3'UTR Luciferase Stable Cell Line

TU118644 1.0 ml Ask for price

Serpinf2 3'UTR Luciferase Stable Cell Line

TU118645 1.0 ml Ask for price

Serping1 3'UTR Luciferase Stable Cell Line

TU118646 1.0 ml Ask for price

Serpinh1 3'UTR Luciferase Stable Cell Line

TU118647 1.0 ml Ask for price

Serpini1 3'UTR Luciferase Stable Cell Line

TU118648 1.0 ml Ask for price

Serpini2 3'UTR Luciferase Stable Cell Line

TU118649 1.0 ml Ask for price

Serpina1 3'UTR Luciferase Stable Cell Line

TU220157 1.0 ml Ask for price

Serpina4 3'UTR Luciferase Stable Cell Line

TU220164 1.0 ml Ask for price

Serpina5 3'UTR Luciferase Stable Cell Line

TU220165 1.0 ml Ask for price

Serpina6 3'UTR Luciferase Stable Cell Line

TU220166 1.0 ml Ask for price

Serpina7 3'UTR Luciferase Stable Cell Line

TU220167 1.0 ml Ask for price

Serpina9 3'UTR Luciferase Stable Cell Line

TU220168 1.0 ml Ask for price

Serpinb2 3'UTR Luciferase Stable Cell Line

TU220174 1.0 ml Ask for price

Serpinb3 3'UTR Luciferase Stable Cell Line

TU220175 1.0 ml Ask for price

Serpinb5 3'UTR Luciferase Stable Cell Line

TU220177 1.0 ml Ask for price

Serpinb7 3'UTR Luciferase Stable Cell Line

TU220180 1.0 ml Ask for price

Serpinb8 3'UTR Luciferase Stable Cell Line

TU220181 1.0 ml Ask for price

Hepa1-6-FLuc cell line with the steady expression of firefly luciferase retains its main properties with promising bioluminescence imaging means.

 

  • Dependable animal fashions are required for the in vivo research of the molecular mechanisms and results of chemotherapeutic medicine in hepatocarcinoma.

 

  • In vivo tracing methods based mostly on firefly luciferase (FLuc) could optimize the non-invasive monitoring of experimental animals. The current research established a murine Hepa1-6-FLuc cell line that stably expressed a retrovirus-delivered FLuc protein gene.

         Rat Angiotensinogen (AGT) ELISA Equipment | Mouse Angiotensinogen (AGT) ELISA Equipment

  • The cell morphology, proliferation, migration and invasion means of Hepa1-6-FLuc cells had been the identical as that of the Hepa1-6 cells, and thus is appropriate to exchange Hepa1-6 cells within the building of hepatocarcinoma animal fashions. No variations in subcutaneous tumor mass and its pathomorphology from implanted Hepa1-6-FLuc cells had been noticed in contrast with Hepa1-6 management tumors.

AGT Antibody

E19-7976-1 50ug/50ul
EUR 145
Description: Available in various conjugation types.

AGT Antibody

E19-7976-2 100ug/100ul
EUR 225
Description: Available in various conjugation types.

AGT Antibody

E10-20360 100μg/100μl
EUR 225
Description: Available in various conjugation types.

AGT Antibody

E312819 200ul
EUR 275
Description: Available in various conjugation types.

AGT Antibody

E92817 100μg
EUR 255
Description: Available in various conjugation types.

AGT Antibody

E91302 100μg
EUR 255
Description: Available in various conjugation types.

AGT Antibody

DF7976 200ul
EUR 420

AGT Antibody

DF7976-100ul 100ul
EUR 280

AGT Antibody

DF7976-200ul 200ul
EUR 350

AGT Antibody

1-CSB-PA001463EA01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000

AGT Antibody

1-CSB-PA001463ESR1HU
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  • 100ul
  • 50ul
Description: A polyclonal antibody against AGT. Recognizes AGT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

AGT Antibody

1-CSB-PA001463ESR2HU
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  • 100ul
  • 50ul
Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200

AGT Antibody

1-CSB-PA001463GA01HU
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  • Ask for price
  • 150ul
  • 50ul
Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

AGT Antibody

36739 100ul
EUR 319

AGT Antibody

36739-100ul 100ul
EUR 302.4

AGT antibody

70R-14180 100 ug
EUR 519
Description: Affinity purified Rabbit polyclonal AGT antibody

AGT antibody

70R-15631 50 ul
EUR 289
Description: Rabbit polyclonal AGT antibody

AGT antibody

70R-6214 50 ug
EUR 467
Description: Rabbit polyclonal AGT antibody

AGT Antibody

BF0069 200ul
EUR 540

AGT Antibody

BF0069-100ul 100ul
EUR 350

AGT Antibody

BF0069-200ul 200ul
EUR 450

AGT Antibody

BF0069-50ul 50ul
EUR 250

AGT Antibody

ABD7976 100 ug
EUR 525.6

AGT Antibody

ABD8022 100 ug
EUR 525.6

AGT Antibody

ABD8094 100 ug
EUR 525.6

AGT Antibody

1-CSB-PA281661
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  • 100ul
  • 50ul
Description: A polyclonal antibody against AGT. Recognizes AGT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:5000, WB:1:500-1:2000

AGT Antibody

1-CSB-PA090852
  • Ask for price
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  • 100ul
  • 50ul
Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:5000, WB:1:500-1:2000

AGT Antibody

1-CSB-PA10079A0Rb
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  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against AGT. Recognizes AGT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:400, IF:1:50-1:200, IP:1:200-1:2000

AGT Antibody

1-CSB-PA446811
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  • Ask for price
  • 100ul
  • 50ul
Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100

AGT Antibody

1-CSB-PA173097
  • Ask for price
  • Ask for price
  • 100ul
  • 50ul
Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA;ELISA:1:1000-1:2000

AGT Antibody

1-CSB-PA693559
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  • Ask for price
  • 100ul
  • 50ul
Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:1000-1:5000, IHC:1:25-1:100

AGT Antibody

GWB-MP545E 50ug Ask for price

AGT Antibody (CT)

6708-100 each
EUR 379.2

AGT Antibody (NT)

6709-100 each
EUR 379.2

Angiotensinogen (AGT) Antibody

20-abx322250
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  • 100 ul
  • 50 ul

Angiotensinogen (AGT) Antibody

20-abx320213
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  • 100 ul
  • 50 ul

Angiotensinogen (AGT) Antibody

20-abx301357
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Angiotensinogen (AGT) Antibody

20-abx339646
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  • 100 ul
  • 50 ul

Angiotensinogen (AGT) Antibody

20-abx101137
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  • Ask for price
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Angiotensinogen (AGT) Antibody

20-abx101138
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Angiotensinogen (AGT) Antibody

20-abx101139
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Angiotensinogen (AGT) Antibody

20-abx101140
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Angiotensinogen (AGT) Antibody

20-abx211344
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  • Ask for price
  • 100 ul
  • 50 ul

Angiotensinogen (AGT) Antibody

20-abx175400
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  • Ask for price
  • 1 mg
  • 200 ug

Angiotensinogen (AGT) Antibody

20-abx171240
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  • Ask for price
  • 1 mg
  • 200 ug

Angiotensinogen (AGT) Antibody

20-abx214416
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  • 100 ul
  • 50 ul

Angiotensinogen (AGT) Antibody

20-abx128540
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  • Ask for price
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Angiotensinogen (AGT) Antibody

abx230393-100ug 100 ug
EUR 610.8

Angiotensinogen (AGT) Antibody

abx230394-100ug 100 ug
EUR 577.2

Angiotensinogen (AGT) Antibody

abx230395-100ug 100 ug
EUR 577.2

Angiotensinogen (AGT) Antibody

20-abx241726
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  • Ask for price
  • 100 ul
  • 50 ul

Angiotensinogen (AGT) Antibody

20-abx241946
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  • Ask for price
  • 100 ul
  • 50 ul

Angiotensinogen (AGT) Antibody

20-abx241947
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  • Ask for price
  • 100 ul
  • 50 ul

Angiotensinogen (AGT) Antibody

20-abx242324
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  • Ask for price
  • 100 ul
  • 50 ul

Angiotensinogen (AGT) Antibody

20-abx110982
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  • 150 ul
  • 50 ul

Angiotensinogen (AGT) Antibody

20-abx004798
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  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Angiotensinogen (AGT) Antibody

abx010360-100ul 100 ul
EUR 493.2

Angiotensinogen (AGT) Antibody

abx033720-400ul 400 ul
EUR 627.6

Angiotensinogen (AGT) Antibody

abx033720-80l 80 µl
EUR 343.2

Angiotensinogen (AGT) Antibody

abx033721-400ul 400 ul
EUR 627.6

Angiotensinogen (AGT) Antibody

abx033721-80l 80 µl
EUR 343.2

Angiotensinogen (AGT) Antibody

abx010360-100g 100 µg Ask for price

Angiotensinogen (AGT) Antibody

abx010360-10g 10 µg
EUR 362.5

Angiotensinogen (AGT) Antibody

abx010360-200g 200 µg Ask for price

Angiotensinogen (AGT) Antibody

abx230393-100g 100 µg
EUR 350

Angiotensinogen (AGT) Antibody

abx230394-100g 100 µg
EUR 350

Angiotensinogen (AGT) Antibody

abx230395-100g 100 µg
EUR 350

Angiotensinogen (AGT) Antibody

abx242324-96tests 96 tests
EUR 250

Angiotensinogen (AGT) Antibody

abx301357-100g 100 µg
EUR 362.5

Angiotensinogen (AGT) Antibody

abx301357-20g 20 µg
EUR 162.5

Angiotensinogen (AGT) Antibody

abx301357-50g 50 µg
EUR 250

Angiotensinogen (AGT) Antibody

abx128540-100l 100 µl
EUR 250

Angiotensinogen (AGT) Antibody

abx128540-1ml 1 ml
EUR 675

Angiotensinogen (AGT) Antibody

abx128540-200l 200 µl
EUR 312.5

Angiotensinogen (AGT) Antibody

abx110982-100l 100 µl
EUR 612.5

Angiotensinogen (AGT) Antibody

abx101137-100l 100 µl
EUR 250

Angiotensinogen (AGT) Antibody

abx101137-1ml 1 ml
EUR 675

Angiotensinogen (AGT) Antibody

abx101137-200l 200 µl
EUR 312.5

Angiotensinogen (AGT) Antibody

abx101138-100l 100 µl
EUR 262.5

Angiotensinogen (AGT) Antibody

abx101138-1ml 1 ml
EUR 687.5

Angiotensinogen (AGT) Antibody

abx101138-200l 200 µl
EUR 325

Angiotensinogen (AGT) Antibody

abx101139-100l 100 µl
EUR 262.5

Angiotensinogen (AGT) Antibody

abx101139-1ml 1 ml
EUR 725

Angiotensinogen (AGT) Antibody

abx101139-200l 200 µl
EUR 337.5

Angiotensinogen (AGT) Antibody

abx101140-100l 100 µl
EUR 275

Angiotensinogen (AGT) Antibody

abx101140-1ml 1 ml
EUR 750

Angiotensinogen (AGT) Antibody

abx101140-200l 200 µl
EUR 337.5

Angiotensinogen (AGT) Antibody

abx033720-100g 100 µg
EUR 281.25

Angiotensinogen (AGT) Antibody

abx033721-100g 100 µg
EUR 281.25

Angiotensinogen (AGT) Antibody

abx241726-96tests 96 tests
EUR 250

 

  • Bioluminescence imaging indicated that the Luc sign of the Hepa1-6-FLuc cells was constantly strengthened with will increase in tumor mass; nonetheless, the Luc sign of Hepa1-6-AdFLuc turned weaker and finally disappeared throughout tumor improvement.

 

 

  • Subsequently, in contrast with the transient expression by adenovirus, steady expression of the FLuc gene in Hepa1-6 cells could higher replicate cell proliferation and survival in vivo, and supply a dependable supply for the institution of hepatocarcinoma fashions.

 

  • Bioluminescent tumor cell strains are experimental instruments of main significance for most cancers investigation, particularly imaging of tumors in xenografted animals. Secure expression of exogenous luciferase in tumor cells mixed to systemic injection of luciferin supplies a wonderful sign/background ratio for exterior optical imaging. Subsequently, there’s a have to rationalize and pace up the manufacturing of luciferase-positive tumor cell strains consultant of a number of tumor phenotypes.