Strategies for Establishing and Utilizing a Secure Cell Line Expressing Each Gaussia Luciferase and Firefly Luciferase to Display screen for Endoplasmic Reticulum Stress.

 

• Coiled-coil area containing 67 (CCDC67) gene is a tumor suppressor gene that displays a major inhibitory impact on quite a lot of tumors. Our earlier research demonstrated that the upregulation of CCDC67 gene in TPC-1 cells inhibited cell proliferation, migration and invasion, and promoted apoptosis in vitro.

 

• Nevertheless, because of the lack of a appropriate cell software, these outcomes weren’t validated in vivo. Within the current research, a thyroid most cancers cell line with steady expression of CCDC67 and luciferase reporter genes was generated and recognized. Firstly, cDNA clones of the CCDC67 gene had been obtained by reverse transcription utilizing a custom-designed primer.

 

• The outcomes of subsequent electrophoresis evaluation and sequencing revealed that the cDNA clones of CCDC67 gene had been obtained efficiently, with a size of 1,862 bp. The lentiviral vectors, containing the CCDC67, luciferase reporter and puromycin acetyltransferase genes, had been co-transfected with two plasmids that encode lentiviral structural proteins and envelope proteins into 293T cells.

        AFAP1L2 3’UTR Luciferase Secure Cell Line

• Following ultracentrifugation, the titer of lentivirus was decided by ELISA to be 5.0×10⁸ TU/ml. The constructed lentiviral vector was used to transfect TPC-1 thyroid most cancers cells, and stabilization was achieved by puromycin screening. The expression of CCDC67 gene, luciferase exercise and tumorigenic means of the generated cell line had been detected.

 

• Reverse transcription-qPCR outcomes demonstrated that the expression ranges of CCDC67 gene in TPC-1 cells following transfection had been elevated 194,46.782-fold in contrast with these within the unfavourable management group (P<0.01).

 

• A better fluorescence depth was detected within the generated cell line, whereas no detectable fluorescence was noticed in untransfected TPC-1 cells. The tumorigenic means of TPC-1-Luc-Puromycin-CCDC67 cells was verified by bioluminescence imaging and histopathological evaluation utilizing a pulmonary metastasis mannequin.

           Human Angiotensinogen (AGT) ELISA Equipment | Mouse Angiotensinogen (AGT) ELISA Equipment

• These outcomes demonstrated {that a} thyroid most cancers cell line with steady expression of CCDC67 and luciferase reporter genes was generated efficiently. The TPC-1-Luc-Puromycin-CCDC67 cell line could also be a useful software for additional analysis on CCDC67 in vivo.

Serpine2 3'UTR Luciferase Stable Cell Line
TU220187	ABM	1.0 ml	Ask for price

SERPINE1 3'UTR Luciferase Stable Cell Line
TU022971	ABM	1.0 ml	EUR 1825.2

SERPINE3 3'UTR Luciferase Stable Cell Line
TU022973	ABM	1.0 ml	EUR 1672.8

Serpine1 3'UTR Luciferase Stable Cell Line
TU118641	ABM	1.0 ml	Ask for price

Serpine3 3'UTR Luciferase Stable Cell Line
TU118643	ABM	1.0 ml	Ask for price

Serpine1 3'UTR Luciferase Stable Cell Line
TU220186	ABM	1.0 ml	Ask for price

Serpine3 3'UTR Luciferase Stable Cell Line
TU220188	ABM	1.0 ml	Ask for price

SERPINA1 3'UTR Luciferase Stable Cell Line
TU022943	ABM	1.0 ml	EUR 1825.2

SERPINA2 3'UTR Luciferase Stable Cell Line
TU022944	ABM	1.0 ml	EUR 2799.6

SERPINA3 3'UTR Luciferase Stable Cell Line
TU022945	ABM	1.0 ml	EUR 1825.2

SERPINA4 3'UTR Luciferase Stable Cell Line
TU022946	ABM	1.0 ml	EUR 1672.8

SERPINA5 3'UTR Luciferase Stable Cell Line
TU022947	ABM	1.0 ml	EUR 1672.8

SERPINA6 3'UTR Luciferase Stable Cell Line
TU022948	ABM	1.0 ml	EUR 1672.8

SERPINA7 3'UTR Luciferase Stable Cell Line
TU022949	ABM	1.0 ml	EUR 1672.8

SERPINA9 3'UTR Luciferase Stable Cell Line
TU022950	ABM	1.0 ml	EUR 1672.8

SERPINB1 3'UTR Luciferase Stable Cell Line
TU022955	ABM	1.0 ml	EUR 1672.8

SERPINB2 3'UTR Luciferase Stable Cell Line
TU022956	ABM	1.0 ml	EUR 1672.8

SERPINB3 3'UTR Luciferase Stable Cell Line
TU022957	ABM	1.0 ml	EUR 1672.8

SERPINB4 3'UTR Luciferase Stable Cell Line
TU022958	ABM	1.0 ml	EUR 1672.8

SERPINB5 3'UTR Luciferase Stable Cell Line
TU022959	ABM	1.0 ml	EUR 1672.8

SERPINB6 3'UTR Luciferase Stable Cell Line
TU022960	ABM	1.0 ml	EUR 2799.6

SERPINB7 3'UTR Luciferase Stable Cell Line
TU022961	ABM	1.0 ml	EUR 1672.8

SERPINB8 3'UTR Luciferase Stable Cell Line
TU022962	ABM	1.0 ml	EUR 1825.2

SERPINB9 3'UTR Luciferase Stable Cell Line
TU022964	ABM	1.0 ml	EUR 1825.2

SERPINC1 3'UTR Luciferase Stable Cell Line
TU022969	ABM	1.0 ml	EUR 1672.8

SERPIND1 3'UTR Luciferase Stable Cell Line
TU022970	ABM	1.0 ml	EUR 1672.8

SERPINF1 3'UTR Luciferase Stable Cell Line
TU022974	ABM	1.0 ml	EUR 1672.8

SERPINF2 3'UTR Luciferase Stable Cell Line
TU022975	ABM	1.0 ml	EUR 1672.8

SERPING1 3'UTR Luciferase Stable Cell Line
TU022976	ABM	1.0 ml	EUR 2799.6

SERPINH1 3'UTR Luciferase Stable Cell Line
TU022977	ABM	1.0 ml	EUR 1672.8

SERPINI1 3'UTR Luciferase Stable Cell Line
TU022979	ABM	1.0 ml	EUR 1672.8

SERPINI2 3'UTR Luciferase Stable Cell Line
TU022980	ABM	1.0 ml	EUR 1672.8

Serpina5 3'UTR Luciferase Stable Cell Line
TU118608	ABM	1.0 ml	Ask for price

Serpina6 3'UTR Luciferase Stable Cell Line
TU118609	ABM	1.0 ml	Ask for price

Serpina7 3'UTR Luciferase Stable Cell Line
TU118610	ABM	1.0 ml	Ask for price

Serpina9 3'UTR Luciferase Stable Cell Line
TU118611	ABM	1.0 ml	Ask for price

Serpinb2 3'UTR Luciferase Stable Cell Line
TU118619	ABM	1.0 ml	Ask for price

Serpinb5 3'UTR Luciferase Stable Cell Line
TU118624	ABM	1.0 ml	Ask for price

Serpinb7 3'UTR Luciferase Stable Cell Line
TU118630	ABM	1.0 ml	Ask for price

Serpinb8 3'UTR Luciferase Stable Cell Line
TU118631	ABM	1.0 ml	Ask for price

Serpinb9 3'UTR Luciferase Stable Cell Line
TU118632	ABM	1.0 ml	Ask for price

Serpinc1 3'UTR Luciferase Stable Cell Line
TU118639	ABM	1.0 ml	Ask for price

Serpind1 3'UTR Luciferase Stable Cell Line
TU118640	ABM	1.0 ml	Ask for price

Serpinf1 3'UTR Luciferase Stable Cell Line
TU118644	ABM	1.0 ml	Ask for price

Serpinf2 3'UTR Luciferase Stable Cell Line
TU118645	ABM	1.0 ml	Ask for price

Serping1 3'UTR Luciferase Stable Cell Line
TU118646	ABM	1.0 ml	Ask for price

Serpinh1 3'UTR Luciferase Stable Cell Line
TU118647	ABM	1.0 ml	Ask for price

Serpini1 3'UTR Luciferase Stable Cell Line
TU118648	ABM	1.0 ml	Ask for price

Serpini2 3'UTR Luciferase Stable Cell Line
TU118649	ABM	1.0 ml	Ask for price

Serpina1 3'UTR Luciferase Stable Cell Line
TU220157	ABM	1.0 ml	Ask for price

Serpina4 3'UTR Luciferase Stable Cell Line
TU220164	ABM	1.0 ml	Ask for price

Serpina5 3'UTR Luciferase Stable Cell Line
TU220165	ABM	1.0 ml	Ask for price

Serpina6 3'UTR Luciferase Stable Cell Line
TU220166	ABM	1.0 ml	Ask for price

Serpina7 3'UTR Luciferase Stable Cell Line
TU220167	ABM	1.0 ml	Ask for price

Serpina9 3'UTR Luciferase Stable Cell Line
TU220168	ABM	1.0 ml	Ask for price

Serpinb2 3'UTR Luciferase Stable Cell Line
TU220174	ABM	1.0 ml	Ask for price

Serpinb3 3'UTR Luciferase Stable Cell Line
TU220175	ABM	1.0 ml	Ask for price

Serpinb5 3'UTR Luciferase Stable Cell Line
TU220177	ABM	1.0 ml	Ask for price

Serpinb7 3'UTR Luciferase Stable Cell Line
TU220180	ABM	1.0 ml	Ask for price

Serpinb8 3'UTR Luciferase Stable Cell Line
TU220181	ABM	1.0 ml	Ask for price

Hepa1-6-FLuc cell line with the steady expression of firefly luciferase retains its main properties with promising bioluminescence imaging means.

 

• Dependable animal fashions are required for the in vivo research of the molecular mechanisms and results of chemotherapeutic medicine in hepatocarcinoma.

 

• In vivo tracing methods based mostly on firefly luciferase (FLuc) could optimize the non-invasive monitoring of experimental animals. The current research established a murine Hepa1-6-FLuc cell line that stably expressed a retrovirus-delivered FLuc protein gene.

         Rat Angiotensinogen (AGT) ELISA Equipment | Mouse Angiotensinogen (AGT) ELISA Equipment

• The cell morphology, proliferation, migration and invasion means of Hepa1-6-FLuc cells had been the identical as that of the Hepa1-6 cells, and thus is appropriate to exchange Hepa1-6 cells within the building of hepatocarcinoma animal fashions. No variations in subcutaneous tumor mass and its pathomorphology from implanted Hepa1-6-FLuc cells had been noticed in contrast with Hepa1-6 management tumors.

AGT Antibody
1-CSB-PA982509	Cusabio	Ask for price Ask for price	100ul 50ul
Description: A polyclonal antibody against AGT. Recognizes AGT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:1000-1:2000, WB:1:200-1:1000

AGT Antibody
E036739	EnoGene	100μg/100μl	EUR 255
Description: Available in various conjugation types.

AGT Antibody
E10-20360	EnoGene	100μg/100μl	EUR 225
Description: Available in various conjugation types.

AGT Antibody
E19-7976-1	EnoGene	50ug/50ul	EUR 145
Description: Available in various conjugation types.

AGT Antibody
E19-7976-2	EnoGene	100ug/100ul	EUR 225
Description: Available in various conjugation types.

AGT Antibody
DF7976	Affbiotech	200ul	EUR 420

AGT Antibody
DF7976-100ul	Affinity Biosciences	100ul	EUR 280

AGT Antibody
DF7976-200ul	Affinity Biosciences	200ul	EUR 350

AGT Antibody
E92817	EnoGene	100μg	EUR 255
Description: Available in various conjugation types.

AGT Antibody
E91302	EnoGene	100μg	EUR 255
Description: Available in various conjugation types.

AGT Antibody
E312819	EnoGene	200ul	EUR 275
Description: Available in various conjugation types.

AGT Antibody
BF0069-100ul	Affinity Biosciences	100ul	EUR 350

AGT Antibody
BF0069-200ul	Affinity Biosciences	200ul	EUR 450

AGT Antibody
BF0069-50ul	Affinity Biosciences	50ul	EUR 250

AGT antibody
70R-14180	Fitzgerald	100 ug	EUR 519
Description: Affinity purified Rabbit polyclonal AGT antibody

AGT antibody
70R-15631	Fitzgerald	50 ul	EUR 289
Description: Rabbit polyclonal AGT antibody

AGT antibody
70R-6214	Fitzgerald	50 ug	EUR 467
Description: Rabbit polyclonal AGT antibody

AGT Antibody
1-CSB-PA281661	Cusabio	Ask for price Ask for price	100ul 50ul
Description: A polyclonal antibody against AGT. Recognizes AGT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:5000, WB:1:500-1:2000

AGT Antibody
1-CSB-PA090852	Cusabio	Ask for price Ask for price	100ul 50ul
Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:5000, WB:1:500-1:2000

AGT Antibody
1-CSB-PA10079A0Rb	Cusabio	Ask for price Ask for price	100ug 50ug
Description: A polyclonal antibody against AGT. Recognizes AGT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:400, IF:1:50-1:200, IP:1:200-1:2000

AGT Antibody
1-CSB-PA446811	Cusabio	Ask for price Ask for price	100ul 50ul
Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100

AGT Antibody
1-CSB-PA693559	Cusabio	Ask for price Ask for price	100ul 50ul
Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:1000-1:5000, IHC:1:25-1:100

AGT Antibody
1-CSB-PA173097	Cusabio	Ask for price Ask for price	100ul 50ul
Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA;ELISA:1:1000-1:2000

AGT Antibody
1-CSB-PA001463EA01HU	Cusabio	Ask for price Ask for price	100ug 50ug
Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000

AGT Antibody
1-CSB-PA001463ESR1HU	Cusabio	Ask for price Ask for price	100ul 50ul
Description: A polyclonal antibody against AGT. Recognizes AGT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

AGT Antibody
1-CSB-PA001463ESR2HU	Cusabio	Ask for price Ask for price	100ul 50ul
Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200

AGT Antibody
1-CSB-PA001463GA01HU	Cusabio	Ask for price Ask for price	150ul 50ul
Description: A polyclonal antibody against AGT. Recognizes AGT from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

AGT Antibody
ABD7976	Nova Lifetech	100ug	EUR 325

AGT Antibody
ABD8022	Nova Lifetech	100ug	EUR 325

AGT Antibody
ABD8094	Nova Lifetech	100ug	EUR 325

AGT Antibody
GWB-MP545E	GenWay Biotech	50ug	Ask for price

AGT antibody
BF0069	Nova Lifetech	100ug	EUR 355

AGT Antibody
MBS7136608-005mL	MyBiosource	0.05mL	EUR 220

AGT Antibody
MBS7136608-01mL	MyBiosource	0.1mL	EUR 300

AGT Antibody
MBS7136608-5x01mL	MyBiosource	5x0.1mL	EUR 1350

AGT Antibody
MBS7109791-005mg	MyBiosource	0.05mg	EUR 190

AGT Antibody
MBS7109791-01mg	MyBiosource	0.1mg	EUR 270

AGT Antibody
MBS7109791-5x01mg	MyBiosource	5x0.1mg	EUR 1205

AGT Antibody
MBS7127834-005mL	MyBiosource	0.05mL	EUR 190

AGT Antibody
MBS7127834-01mL	MyBiosource	0.1mL	EUR 270

AGT Antibody
MBS7127834-5x01mL	MyBiosource	5x0.1mL	EUR 1205

AGT Antibody
MBS7127835-005mL	MyBiosource	0.05mL	EUR 190

AGT Antibody
MBS7127835-01mL	MyBiosource	0.1mL	EUR 270

AGT Antibody
MBS7127835-5x01mL	MyBiosource	5x0.1mL	EUR 1205

AGT Antibody
MBS7127836-005mL	MyBiosource	0.05mL	EUR 190

AGT Antibody
MBS7127836-01mL	MyBiosource	0.1mL	EUR 270

AGT Antibody
MBS7127836-5x01mL	MyBiosource	5x0.1mL	EUR 1205

AGT Antibody
MBS7127837-005mL	MyBiosource	0.05mL	EUR 190

AGT Antibody
MBS7127837-01mL	MyBiosource	0.1mL	EUR 270

AGT Antibody
MBS7127837-5x01mL	MyBiosource	5x0.1mL	EUR 1205

AGT Antibody
MBS7127838-005mL	MyBiosource	0.05mL	EUR 190

AGT Antibody
MBS7127838-01mL	MyBiosource	0.1mL	EUR 270

AGT Antibody
MBS7127838-5x01mL	MyBiosource	5x0.1mL	EUR 1205

AGT Antibody
MBS7126806-005mL	MyBiosource	0.05mL	EUR 190

AGT Antibody
MBS7126806-01mL	MyBiosource	0.1mL	EUR 270

AGT Antibody
MBS7126806-5x01mL	MyBiosource	5x0.1mL	EUR 1205

AGT Antibody
MBS7126807-005mL	MyBiosource	0.05mL	EUR 190

AGT Antibody
MBS7126807-01mL	MyBiosource	0.1mL	EUR 270

AGT Antibody
MBS7126807-5x01mL	MyBiosource	5x0.1mL	EUR 1205

AGT antibody
MBS9402140-005mL	MyBiosource	0.05mL	EUR 300

AGT antibody
MBS9402140-01mL	MyBiosource	0.1mL	EUR 390

AGT antibody
MBS9402140-5x01mL	MyBiosource	5x0.1mL	EUR 1610

AGT Antibody
MBS9413904-01mL	MyBiosource	0.1mL	EUR 305

AGT Antibody
MBS9413904-5x01mL	MyBiosource	5x0.1mL	EUR 1230

AGT Antibody
MBS8576462-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

AGT Antibody
MBS8576462-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

AGT Antibody
MBS8576462-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

AGT Antibody
MBS8576462-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

AGT Antibody
MBS8576462-02mL	MyBiosource	0.2mL	EUR 345

AGT Antibody
MBS8504990-01mg	MyBiosource	0.1mg	EUR 325

AGT Antibody
MBS8504990-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

AGT Antibody
MBS8504990-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

AGT Antibody
MBS8504990-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

AGT Antibody
MBS8504990-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

AGT Antibody
MBS850936-01mg	MyBiosource	0.1mg	EUR 305

AGT Antibody
MBS850936-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

AGT Antibody
MBS850936-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

AGT Antibody
MBS850936-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

AGT Antibody
MBS850936-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

AGT Antibody
MBS8517273-005mg	MyBiosource	0.05mg	EUR 235

AGT Antibody
MBS8517273-01mg	MyBiosource	0.1mg	EUR 305

AGT Antibody
MBS8517273-01mLAF405M	MyBiosource	0.1mL(AF405M)	EUR 465

AGT Antibody
MBS8517273-01mLAF546	MyBiosource	0.1mL(AF546)	EUR 465

AGT Antibody
MBS8517273-01mLAF750	MyBiosource	0.1mL(AF750)	EUR 465

AGT Antibody
MBS8501506-01mg	MyBiosource	0.1mg	EUR 325

AGT Antibody
MBS8501506-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

AGT Antibody
MBS8501506-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

AGT Antibody
MBS8501506-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

AGT Antibody
MBS8501506-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

AGT antibody
MBS533865-01mL	MyBiosource	0.1mL	EUR 810

 

• Bioluminescence imaging indicated that the Luc sign of the Hepa1-6-FLuc cells was constantly strengthened with will increase in tumor mass; nonetheless, the Luc sign of Hepa1-6-AdFLuc turned weaker and finally disappeared throughout tumor improvement.

 

 

• Subsequently, in contrast with the transient expression by adenovirus, steady expression of the FLuc gene in Hepa1-6 cells could higher replicate cell proliferation and survival in vivo, and supply a dependable supply for the institution of hepatocarcinoma fashions.

 

• Bioluminescent tumor cell strains are experimental instruments of main significance for most cancers investigation, particularly imaging of tumors in xenografted animals. Secure expression of exogenous luciferase in tumor cells mixed to systemic injection of luciferin supplies a wonderful sign/background ratio for exterior optical imaging. Subsequently, there’s a have to rationalize and pace up the manufacturing of luciferase-positive tumor cell strains consultant of a number of tumor phenotypes.