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Serpin peptidase inhibitor

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Rat Angiotensinogen ELISA Kit

Rat Angiotensinogen ELISA Kit

July 26, 2020 0 By Keith

Activation of the renin-angiotensin system in excessive fructose-induced metabolic syndrome

 

 

  • Excessive fructose consumption induces hyperglycemia and hypertension. Nevertheless, the mechanism by which fructose induces metabolic syndrome is essentially unknown. We hypothesized that top fructose consumption induces activation of the renin-angiotensin system (RAS), leading to hypertension and metabolic syndrome.

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  • We supplied 11-week-old Sprague-Dawley rats with ingesting water, with or with out 20% fructose, for 2 weeks. We measured serum renin, angiotensin II (Ang II), and aldosterone (Aldo) utilizing ELISA kits. The expression of RAS genes was decided by quantitative reverse transcription polymerase chain response.

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  • Excessive fructose consumption elevated physique weight and water retention, no matter meals consumption or urine quantity. After two weeks, fructose consumption induced glucose intolerance and hypertension. Excessive fructose consumption elevated serum renin, Ang II, triglyceride, and levels of cholesterol, however not Aldo ranges.

 

 

  • Excessive fructose consumption elevated the expression of angiotensinogen within the liver; angiotensin-converting enzyme within the lungs; and renin, angiotensin II sort 1a receptor (AT1aR), and angiotensin II sort 1b receptor (AT1bR) within the kidneys. Nevertheless, expression of AT1aR and AT1bR within the adrenal glands didn’t enhance in rats given fructose. Taken collectively, these outcomes point out that top fructose consumption induces activation of RAS, leading to hypertension and metabolic syndrome.
    Key phrases: Fructose; Hypertension; Metabolic syndrome; Weight problems; Renin-angiotensin system.

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×

 

Up-regulation of intrarenal renin-angiotensin system contributes to renal harm in high-salt induced hypertension rats.

 

 

OBJECTIVE
To check the speculation that in a high-salt induced hypertension in regular rats, whether or not the modifications of intrarenal renin-agiotensin system (RAS) play a crucial position in renal harm and might be mirrored by urinary angiotensinogen (AGT).
METHODS
Within the research, 27 normotensive male Wistar-Kyoto rats had been divided into management group [0.3% (mass faction) NaCl in chow, n=9, NS], high-salt food plan group [8% (mass faction) NaCl in chow, n=9, HS] and high-salt food plan with Losartan group [8% (mass faction) NaCl in chow and 20 mg/(kg×d) Losartan in gavages, n=9, HS+L)], and had been fed for six weeks.
The blood stress was monitored and urine samples had been collected each 2 weeks. AGTs in plasma, kidney and urine had been measured by ELISA kits. The renal cortex expression of mRNA and protein of AGT had been measured by Actual-time PCR and immunohistochemistry (IHC). The renin exercise and ANG II had been measured by radioimmunoassay (RIA) kits.

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RESULTS
In contrast with NS, the systolic blood stress (SBP) [(156 ± 2) mmHg vs. (133 ± 3) mmHg, P<0.05] elevated considerably on the finish of the 2nd week, and the urinary protein [(14.07 ± 2.84) mg/24 h vs. (7.62 ± 3.02) mg/24 h, P<0.05] elevated considerably on the finish of the sixth week in HS.
In contrast with HS, there was no important distinction in SBP (P>0.05) however the proteinuria [(9.69 ± 2.73) mg/24 h vs. (14.07 ± 2.84) mg/24 h, P<0.01] decreased considerably in HS+L. In contrast with NS, there was no important distinction within the plasma renin exercise, angiotensinogen and ANG II degree in HS (P>0.05), however the renal cortex renin content material [(8.72 ± 1.98) ng/(mL × h) vs. (4.37 ± 1.26) ng/(mL × h), P<0.05], AGT formation [(4.02 ± 0.60) ng/mg vs. (2.59 ± 0.42) ng/mg, P<0.01], ANG II degree [(313.8 ± 48.76) pmol/L vs. (188.9 ± 46.95) pmol/L, P<0.05] had been elevated considerably in HS, and the urinary AGT and ANG II excretion charges elevated considerably (P<0.05). In contrast with HS, the plasma renin exercise, angiotensinogen and ANG II degree had been considerably elevated (P<0.05), however the renal cortex renin content material, AGT formation, ANG II degree considerably decreased (P<0.05), and the urinary AGT and ANG II excretion charges decreased considerably in HS+L (P<0.05). The urinary AGT excretion charges had been positively correlated with the AGT degree within the renal cortex (P<0.05).
CONCLUSIONS
Up-regulation of intarenal RAS might contribute to renal harm in high-salt induced hypertension rats. Urinary AGT might replicate the standing of intrarenal RAS.

 

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EUR 198

Rat angiotensinogen (aGT) ELISA Kit

1-CSB-E08565r Cusabio
  • EUR 1160.40
  • EUR 7110.00
  • EUR 3760.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each

Rat angiotensinogen,aGT ELISA Kit

CN-01790R1 ChemNorm 96T
EUR 536.4

Rat angiotensinogen,aGT ELISA Kit

CN-01790R2 ChemNorm 48T
EUR 355.2

Rat Agt/ Angiotensinogen ELISA Kit

E0038Ra Sunlong 1 Kit
EUR 685.2

Rat Agt(Angiotensinogen) ELISA Kit

ER0371 FN Test 96T
EUR 628.92

Rat angiotensinogen(aGT)ELISA Kit

GA-E0665RT-48T GenAsia Biotech 48T
EUR 447.6

Rat angiotensinogen(aGT)ELISA Kit

GA-E0665RT-96T GenAsia Biotech 96T
EUR 730.8

Rat Angiotensinogen (AGT) ELISA Kit

SEA797Ra-10x96wellstestplate Cloud-Clone 10x96-wells test plate
EUR 5552.14

Rat Angiotensinogen (AGT) ELISA Kit

SEA797Ra-1x48wellstestplate Cloud-Clone 1x48-wells test plate
EUR 562.42

Rat Angiotensinogen (AGT) ELISA Kit

SEA797Ra-1x96wellstestplate Cloud-Clone 1x96-wells test plate
EUR 752.02

Rat Angiotensinogen (AGT) ELISA Kit

SEA797Ra-5x96wellstestplate Cloud-Clone 5x96-wells test plate
EUR 3024.07

Rat Angiotensinogen (AGT) ELISA Kit

4-SEA797Ra Cloud-Clone
  • EUR 5612.40
  • EUR 2965.20
  • EUR 752.40
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells

Rat angiotensinogen(aGT)ELISA Kit

QY-E10987 Qayee Biotechnology 96T
EUR 433.2

Rat Angiotensinogen ELISA Kit (AGT)

RK03477 Abclonal 96 Tests
EUR 625.2

Rat angiotensinogen,aGT ELISA Kit

YLA0162RA-48T Shanghai YL Biotech 48T
EUR 465

Rat angiotensinogen,aGT ELISA Kit

YLA0162RA-96T Shanghai YL Biotech 96T
EUR 600

ELISA kit for Rat AGT (Angiotensinogen)

ELK4678 ELK Biotech 1 plate of 96 wells
EUR 518.4

ELISA kit for Rat AGT (Angiotensinogen)

E-EL-R0199 Elabscience Biotech 1 plate of 96 wells
EUR 640.8

Agt ELISA Kit| Rat Angiotensinogen ELISA Kit

EF017231 Lifescience Market 96 Tests
EUR 826.8

Rat Angiotensinogen (AGT) CLIA Kit

20-abx492048 Abbexa
  • EUR 10282.80
  • EUR 5472.00
  • EUR 1262.40
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Angiotensinogen (AGT) CLIA Kit

abx195130-96tests Abbexa 96 tests
EUR 990

Rat Angiotensinogen (AGT) Protein

20-abx065353 Abbexa
  • EUR 693.60
  • EUR 309.60
  • EUR 2064.00
  • EUR 828.00
  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Angiotensinogen (AGT) Protein

20-abx651468 Abbexa
  • EUR 794.40
  • EUR 326.40
  • EUR 2414.40
  • EUR 944.40
  • EUR 577.20
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Angiotensinogen (AGT) ELISA Kit

20-abx150656 Abbexa
  • EUR 7970.40
  • EUR 4250.40
  • EUR 990.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Angiotensinogen (AGT) ELISA Kit

20-abx153638 Abbexa
  • EUR 7970.40
  • EUR 4250.40
  • EUR 990.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Angiotensinogen (AGT) ELISA Kit

abx253518-96tests Abbexa 96 tests
EUR 801.6

Mouse Angiotensinogen (AGT) ELISA Kit

abx254811-96tests Abbexa 96 tests
EUR 801.6

Chicken Angiotensinogen (AGT) ELISA Kit

abx354753-96tests Abbexa 96 tests
EUR 990

Monkey Angiotensinogen (AGT) ELISA Kit

abx354991-96tests Abbexa 96 tests
EUR 990

Pig Angiotensinogen (AGT) ELISA Kit

abx355132-96tests Abbexa 96 tests
EUR 990

Rabbit Angiotensinogen (AGT) ELISA Kit

abx355228-96tests Abbexa 96 tests
EUR 990

Sheep Angiotensinogen (AGT) ELISA Kit

abx355368-96tests Abbexa 96 tests
EUR 1111.2

Cow Angiotensinogen (AGT) ELISA Kit

abx514023-96tests Abbexa 96 tests
EUR 1093.2

Mouse Angiotensinogen (AGT) ELISA Kit

abx573245-96tests Abbexa 96 tests
EUR 858

Human Angiotensinogen (AGT) ELISA Kit

abx573778-96tests Abbexa 96 tests
EUR 801.6

Human angiotensinogen,aGT ELISA Kit

201-12-1227 SunredBio 96 tests
EUR 528

Human angiotensinogen (aGT) ELISA Kit

CSB-E08564h-24T Cusabio 1 plate of 24 wells
EUR 198

Human angiotensinogen (aGT) ELISA Kit

1-CSB-E08564h Cusabio
  • EUR 1080.00
  • EUR 6571.20
  • EUR 3480.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each

Mouse angiotensinogen (aGT) ELISA Kit

CSB-E08566m-24T Cusabio 1 plate of 24 wells
EUR 198

Mouse angiotensinogen (aGT) ELISA Kit

1-CSB-E08566m Cusabio
  • EUR 1135.20
  • EUR 6938.40
  • EUR 3672.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each

Mouse angiotensinogen,aGT ELISA Kit

CN-02669M1 ChemNorm 96T
EUR 560.4

Mouse angiotensinogen,aGT ELISA Kit

CN-02669M2 ChemNorm 48T
EUR 379.2

Human angiotensinogen,aGT ELISA Kit

CN-03909H1 ChemNorm 96T
EUR 542.4

Human angiotensinogen,aGT ELISA Kit

CN-03909H2 ChemNorm 48T
EUR 362.4

Mouse Agt/ Angiotensinogen ELISA Kit

E0056Mo Sunlong 1 Kit
EUR 685.2

Human AGT/ Angiotensinogen ELISA Kit

E0087Hu Sunlong 1 Kit
EUR 644.4

Human AGT(Angiotensinogen ) ELISA Kit

EH0550 FN Test 96T
EUR 628.92

Mouse Agt(Angiotensinogen) ELISA Kit

EM0458 FN Test 96T
EUR 628.92

Human angiotensinogen(aGT)ELISA Kit

GA-E1243HM-48T GenAsia Biotech 48T
EUR 346.8

Human angiotensinogen(aGT)ELISA Kit

GA-E1243HM-96T GenAsia Biotech 96T
EUR 559.2

Mouse angiotensinogen(aGT)ELISA Kit    

GA-E0649MS-48T GenAsia Biotech 48T
EUR 403.2

Mouse angiotensinogen(aGT)ELISA Kit    

GA-E0649MS-96T GenAsia Biotech 96T
EUR 640.8

Human Angiotensinogen, AGT ELISA KIT

ELI-02698h Lifescience Market 96 Tests
EUR 988.8

Mouse Angiotensinogen, Agt ELISA KIT

ELI-02699m Lifescience Market 96 Tests
EUR 1038

Bovine Angiotensinogen, AGT ELISA KIT

ELI-02700b Lifescience Market 96 Tests
EUR 1113.6

Chicken Angiotensinogen, AGT ELISA KIT

ELI-02701c Lifescience Market 96 Tests
EUR 1113.6

Human Angiotensinogen (AGT) ELISA Kit

SEA797Hu-10x96wellstestplate Cloud-Clone 10x96-wells test plate
EUR 5128.02

Human Angiotensinogen (AGT) ELISA Kit

SEA797Hu-1x48wellstestplate Cloud-Clone 1x48-wells test plate
EUR 527.48

Human Angiotensinogen (AGT) ELISA Kit

SEA797Hu-1x96wellstestplate Cloud-Clone 1x96-wells test plate
EUR 702.12

Human Angiotensinogen (AGT) ELISA Kit

SEA797Hu-5x96wellstestplate Cloud-Clone 5x96-wells test plate
EUR 2799.54

Human Angiotensinogen (AGT) ELISA Kit

4-SEA797Hu Cloud-Clone
  • EUR 5188.80
  • EUR 2739.60
  • EUR 703.20
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells

Mouse Angiotensinogen (AGT) ELISA Kit

SEA797Mu-10x96wellstestplate Cloud-Clone 10x96-wells test plate
EUR 5269.39

Mouse Angiotensinogen (AGT) ELISA Kit

SEA797Mu-1x48wellstestplate Cloud-Clone 1x48-wells test plate
EUR 539.12

Mouse Angiotensinogen (AGT) ELISA Kit

SEA797Mu-1x96wellstestplate Cloud-Clone 1x96-wells test plate
EUR 718.75

Mouse Angiotensinogen (AGT) ELISA Kit

SEA797Mu-5x96wellstestplate Cloud-Clone 5x96-wells test plate
EUR 2874.38

Mouse Angiotensinogen (AGT) ELISA Kit

4-SEA797Mu Cloud-Clone
  • EUR 5330.40
  • EUR 2815.20
  • EUR 718.80
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells

Human Angiotensinogen ELISA Kit (AGT)

RK00848 Abclonal 96 Tests
EUR 625.2

Human angiotensinogen(aGT)ELISA Kit

QY-E00720 Qayee Biotechnology 96T
EUR 433.2

Mouse angiotensinogen(aGT)ELISA Kit

QY-E20099 Qayee Biotechnology 96T
EUR 433.2

Mouse Angiotensinogen ELISA Kit (AGT)

RK02579 Abclonal 96 Tests
EUR 625.2

Canine angiotensinogen(aGT)ELISA Kit

QY-E70099 Qayee Biotechnology 96T
EUR 511.2

Mouse angiotensinogen,aGT ELISA Kit    

YLA0955MO-48T Shanghai YL Biotech 48T
EUR 465

Mouse angiotensinogen,aGT ELISA Kit    

YLA0955MO-96T Shanghai YL Biotech 96T
EUR 600

Human angiotensinogen,aGT ELISA Kit

YLA0062HU-48T Shanghai YL Biotech 48T
EUR 435

Human angiotensinogen,aGT ELISA Kit

YLA0062HU-96T Shanghai YL Biotech 96T
EUR 562.5

Angiotensinogen (AGT) Antibody

20-abx171240 Abbexa
  • EUR 944.40
  • EUR 493.20
  • 1 mg
  • 200 ug
×

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  • PCR ELISA sites
  • EPIQUIK
  • Involvement of a Membrane-Bound Amphiphilic Helix in Substrate Discrimination and Binding by an Escherichia coli S2P Peptidase RseP
  • 3-point major cardiovascular event outcome for patients with T2D treated with dipeptidyl peptidase-4 inhibitor or glucagon-like peptide-1 receptor agonist in addition to metformin monotherapy
  • The Inhibitory Effect of 6-Gingerol on Ubiquitin-Specific Peptidase 14 Enhances Autophagy-Dependent Ferroptosis and Anti-Tumor in vivo and in vitro

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